Further bioinformatics and IHC analysis showed that miR577/TESC is the likely downstream signaling pathway for hsa_circ_0001675. Bioinformatics was used to construct a ceRNA network of hsa_circ_0001675 with six differentially expressed miRNAs (hsa-miR-330-5p, hsa-miR-498, hsa-miR-532-3p, hsa-miR-577, hsa-miR-1248, and hsa-miR-1305) and 411 differentially expressed mRNAs and found that the neuroactive ligand-receptor interaction, and the cAMP and calcium signaling pathways were particularly enriched. Low hsa_circ_0001675 expression also correlated with the overall survival (OS) rate and the progression-free survival (PFS) rate of HNC patients ( P < 0.001). Low hsa_circ_0001675 expression was positively associated with tumor invasion and clinical staging ( P < 0.05), and its area under the ROC curve (AUC) was 0.7776. hsa_circ_0001675 had significantly lower expression in HNC than in normal tissue ( P < 0.01). mRNA expression was verified by immunohistochemistry (IHC).Ī total of 714 differentially expressed circRNAs were detected in HNC, and the low expression of hsa_circ_0001675 was particularly significant (fold change = -4.85, P = 6.305E-05). Bioinformatics was used to find target circRNAs that acted as competitive endogenous RNA (ceRNA) and construct a circRNA-miRNA-mRNA regulatory network. This data was combined with clinicopathological information to analyze the diagnostic value of target circRNA. In 103 HNC and adjacent normal tissues, real-time fluorescent quantitative PCR (qRT-PCR) was used to verify the differential expression of target circRNAs. Target circRNAs with significantly differential expression were obtained. High-throughput sequencing was used to analyze circRNA expression in 18 matched HNC and adjacent normal tissues. MindMup also integrates with Office365 and Google authentication for easy account management.This study sought to identify circular RNAs (circRNA) that participate in the regulation of head and neck cancer (HNC), analyze their clinical application, and predict their molecular mechanism during HNC. Administer easilyĪdministrators can easily control sharing settings for the entire organisation. Quickly add images and icons to your maps to provide context and illustrations. MindMup for Google Drive also allows you to link to other project documents on Google Drive easily. Attach documentsĪdd longer text, videos and spreadsheets to your maps. Maps saved to MindMup Cloud support concurrent editing for teams and classrooms, enabling you to instantly see updates made to your map by other users. Manage project progress easily with icons and priorities. MindMup Atlas automatically adds images and contextual information to ensure that your maps show up nicely on Twitter, Facebook, Google+, LinkedIn and Pinterest.Īdd measurements to map nodes to automatically calculate cost, completion and other important factors. Use a frictionless planning canvas to capture and organise your thoughts, and then put them into a powerful storyboard to turn a hierarchy of ideas into a sequence of slides. Use notes and attachments to quickly create a structure for an article or a writing assignment, then create a document outline and continue editing in your favourite writing tool Create storyboards
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |